Nutrition & Metabolism Center, Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609-1673, USA. Methylene blue (MB) has been used clinically for about a century to treat numerous ailments. We show that MB and other diaminophenothiazines extend the life span of human IMR90 fibroblasts in tissue culture by >20 population doubling (PDLs). MB delays senescence at nM levels in IMR90 by enhancing mitochondrial function. MB increases mitochondrial complex IV by 30%, enhances cellular oxygen consumption by 37-70%, increases heme synthesis, and reverses premature senescence caused by H2O2 or cadmium. MB also induces phase-2 antioxidant enzymes in hepG2 cells. Flavin-dependent enzymes are known to use NAD(P)H to reduce MB to leucomethylene blue (MBH2), whereas cytochrome c reoxidizes MBH2 to MB. Experiments on lysates from rat liver mitochondria suggest the ratio MB/cytochrome c is important for the protective actions of MB. We propose that the cellular senescence delay caused by MB is due to cycling between MB and MBH2 in mitochondria, which may partly explain the increase in specific mitochondrial activities. Cycling of MB between oxidized and reduced forms may block oxidant production by mitochondria. Mitochondrial dysfunction and oxidative stress are thought to be key aberrations that lead to cellular senescence and aging. MB may be useful to delay mitochondrial dysfunction with aging and the decrease in complex IV in Alzheimer disease.
> Nutrition & Metabolism Center, Children's Hospital Oakland Research > Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609-1673, USA. > Methylene blue (MB) has been used clinically for about a century to > treat numerous ailments. We show that MB and other diaminophenothiazines > extend the life span of human IMR90 fibroblasts in tissue culture by >20 > population doubling (PDLs). MB delays senescence at nM levels in IMR90 > by enhancing mitochondrial function. MB increases mitochondrial complex > IV by 30%, enhances cellular oxygen consumption by 37-70%, increases > heme synthesis, and reverses premature senescence caused by H2O2 or > cadmium. MB also induces phase-2 antioxidant enzymes in hepG2 cells. > Flavin-dependent enzymes are known to use NAD(P)H to reduce MB to > leucomethylene blue (MBH2), whereas cytochrome c reoxidizes MBH2 to MB. > Experiments on lysates from rat liver mitochondria suggest the ratio > MB/cytochrome c is important for the protective actions of MB. We > propose that the cellular senescence delay caused by MB is due to > cycling between MB and MBH2 in mitochondria, which may partly explain > the increase in specific mitochondrial activities. Cycling of MB between > oxidized and reduced forms may block oxidant production by mitochondria. > Mitochondrial dysfunction and oxidative stress are thought to be key > aberrations that lead to cellular senescence and aging. MB may be useful > to delay mitochondrial dysfunction with aging and the decrease in > complex IV in Alzheimer disease.
> PMID: 17928358
hmm, sounds interesting, but what reaction would one get with the white of the eyes turned blue....? ;-)
methylene blue eye whites - sounds like something from Dune. The spice extended life and gave those who used it blued overall eyes. Sci fi predicts the future? If it extends life, everyone would use it.
On Aug 24, 11:05 am, windwatcher <windwatc...@mac.com> wrote:
> methylene blue eye whites - sounds like something from Dune. The spice > extended life and gave those who used it blued overall eyes. Sci fi > predicts the future? If it extends life, everyone would use it.
I wonder if the extremely diluted MB they describe is really what they used in their experiments. This could have been stated in order to prevent ordinary citizens from going out and buying MB and trying it out. At such diluted levels as described in their paper, no one is going to hurt themselves. And certainly, no one is going to end up with blue eyed eyeballs at such an extremely low concentration.
The concentrations as described in the Science Daily article - a few rain drops diluted in two olympic sized pools, are what apparently was applied to the "petri dish" samples of living cells. In order to gain such a concentration in one's blood stream, one would obviously need a higher ingested concentration. How much so I do not know. Anyone have an idea? if one weighs 70kg and a human is 60% water by weight, then there is 42 liters of water in one's body. This could be used to reach a rough estimate of what one would need to ingest to reach the concentrations described in the paper, however, the MG would not necessarily be distributed evenly throughout the 42 liters of water within one's body.
First question to ask - is this quackery that was reported in Science Daily? Who are these reserachers? Reputable or is this a biomedical instance of Cold Fusion research?
> On Aug 24, 11:05 am, windwatcher <windwatc...@mac.com> wrote:
> > methylene blue eye whites - sounds like something from Dune. The spice > > extended life and gave those who used it blued overall eyes. Sci fi > > predicts the future? If it extends life, everyone would use it.
> I wonder if the extremely diluted MB they describe is really what they > used in their experiments. This could have been stated in order to > prevent ordinary citizens from going out and buying MB and trying it > out. At such diluted levels as described in their paper, no one is > going to hurt themselves. And certainly, no one is going to end up > with blue eyed eyeballs at such an extremely low concentration.
> The concentrations as described in the Science Daily article - a few > rain drops diluted in two olympic sized pools, are what apparently was > applied to the "petri dish" samples of living cells. In order to gain > such a concentration in one's blood stream, one would obviously need a > higher ingested concentration. How much so I do not know. Anyone have > an idea? if one weighs 70kg and a human is 60% water by weight, then > there is 42 liters of water in one's body. This could be used to reach > a rough estimate of what one would need to ingest to reach the > concentrations described in the paper, however, the MG would not > necessarily be distributed evenly throughout the 42 liters of water > within one's body.
I've used this for quite a while. Dosage : Mix 0.1cc scoop (~40 mg) methylene blue in 30 ml (1 oz) dropper bottle of water. Delivers ~50 mcg/drop. I mix one drop in each of my three tea drinks, and one in the morning early - so 200 mcg/day total. Patent app info and excerpts follows. JLC
Diaminophenothiazine compositions and uses thereof
In one embodiment, the dosage is between 0.1-10.0 mcg/kg body weight/ day. In a preferred embodiment, the dosage achieves a blood concentration of about 1.0 to 100 nM of the diaminophenothiazine. In another embodiment, the dosage achieves a brain concentration of about 1.0 to 100 nM of the diaminophenothiazine. For a human weighing about 160 lbs and having about 5 liters of blood, the daily dose of methylene blue (mol. wt.=319) needed to achieve this concentration is about 1.6 to 160 mcg. The oral dosage is typically given one to three times daily for prolonged periods, usually for at least 30 days. In some cases, the oral dosage is administered chronically, i.e. regularly over a period of at least 3, preferably at least 6, more preferably at least 12 months, wherein the regularity is at least once, preferably at least 2-3 times, more preferably at least 7 times (daily) per week.
Long-Term Administration of diaminophenothiazines Improve Skin Elasticity and Activity Levels in Patients over 50.
A double blind, randomized, vehicle controlled study is conducted in 160 subjects ranging in ages from 50 to 65. Treatment groups are prescribed 25 mcg diaminophenothiazine (azure A, azure B, azure C, thionine, toluidine blue, methylene blue, new methylene blue, or 1-9-dimethyl methylene blue) tablets taken orally with meals 3 times daily. Clinical monitoring, subjective self assessment, objective measurement methods of skin elasticity, epidermal hydration and skin surface lipids are used to determine effects of each treatment at four visits during 24 weeks. Clinical monitoring includes wrinkle counts, measurement of wrinkle depth around the right eye, and nasolabial fold depth. Results demonstrate consistent efficacy of diaminophenothiazine treatment over placebo in counteracting different signs of aging in the skin and improving overall energy levels.
I'm assessing the concentrations you state. Lets start with the first one below. A Standard medical "Drop" is about 83 micro-liters. Divide that drop by 30 milli-liters and then multiply by 40 mg and the result is 110 mcg of M-B per drop. This is twice the concentration you state. Please review and restate the calculation if in error. Using 60 micro- liters per drop results in delivery of 80 mcg of MB per drop. Even that is 50% more than what you state.
> I've used this for quite a while. Dosage : > Mix 0.1cc scoop (~40 mg) methylene blue in 30 ml (1 oz) dropper bottle > of water. Delivers ~50 mcg/drop. > I mix one drop in each of my three tea drinks, and one in the morning > early - so 200 mcg/day total. Patent app info and excerpts follows. > JLC
> I'm assessing the concentrations you state. Lets start with the first > one below. A Standard medical "Drop" is about 83 micro-liters. Divide > that drop by 30 milli-liters and then multiply by 40 mg and the result > is 110 mcg of M-B per drop. This is twice the concentration you state. > Please review and restate the calculation if in error. Using 60 micro- > liters per drop results in delivery of 80 mcg of MB per drop. Even > that is 50% more than what you state.
> > I've used this for quite a while. Dosage : > > Mix 0.1cc scoop (~40 mg) methylene blue in 30 ml (1 oz) dropper bottle > > of water. Delivers ~50 mcg/drop. > > I mix one drop in each of my three tea drinks, and one in the morning > > early - so 200 mcg/day total. Patent app info and excerpts follows. > > JLC- Hide quoted text -
> - Show quoted text -
Every drop size is different depending on the viscosity of the fluid and size of the orifice. I measured 26 drops per ml from my dropper with this solution. Your mileage may vary.
40000 mcg divided by 30 ml divided by 26 drops per ml equals 51 mcg/ drop. JLC
> Nutrition & Metabolism Center, Children's Hospital Oakland Research > Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609-1673, USA. > Methylene blue (MB) has been used clinically for about a century to > treat numerous ailments. We show that MB and other diaminophenothiazines > extend the life span of human IMR90 fibroblasts in tissue culture by >20 > population doubling (PDLs). MB delays senescence at nM levels in IMR90 > by enhancing mitochondrial function. MB increases mitochondrial complex > IV by 30%, enhances cellular oxygen consumption by 37-70%, increases > heme synthesis, and reverses premature senescence caused by H2O2 or > cadmium. MB also induces phase-2 antioxidant enzymes in hepG2 cells. > Flavin-dependent enzymes are known to use NAD(P)H to reduce MB to > leucomethylene blue (MBH2), whereas cytochrome c reoxidizes MBH2 to MB. > Experiments on lysates from rat liver mitochondria suggest the ratio > MB/cytochrome c is important for the protective actions of MB. We > propose that the cellular senescence delay caused by MB is due to > cycling between MB and MBH2 in mitochondria, which may partly explain > the increase in specific mitochondrial activities. Cycling of MB between > oxidized and reduced forms may block oxidant production by mitochondria. > Mitochondrial dysfunction and oxidative stress are thought to be key > aberrations that lead to cellular senescence and aging. MB may be useful > to delay mitochondrial dysfunction with aging and the decrease in > complex IV in Alzheimer disease.
> PMID: 17928358
Hello I just read your article pertaining to Methylene blue delaying senescence in the Mitochondria.. I am a registered Pharmacist .. UCSF.. Do you have more information, and what dose, how many drops would an average person of 70 kg take daily? Sincerely, Clayton Tom, RPh
Well that is a pretty small drop in comparison to what the wiki page for a drop states. The wiki page lists several defined drop sizes ranging from 50 to 80-odd micro-liters. I'll have to measure my droppers drop size to determine the concentration to match the dosage you state.
Presently MB is very inexpensive (that could change but apparently its production is not expensive and suppliers could adapt to demand). I do not know if there is a difference in purity between MB sold by lab material supplier intended as a dye, and MB meant for human consumption such as for treatment of methemoglobinemia. Do you have any idea if there is a difference and what impurities would exist in a "lower grade" MB? And would you recommend not using MB sold as a lab stain?
A 1 fluid oz bottle at 1% (w/v) would need to be diluted by a factor of about 7. The MB is at a low concentration. Any impurities would be at a lower level. The toxicity of MB alone, using the Wikipedia reference, appears to be on the order of 5 mg/kg of body weight. Such a dosage is 5,000 times greater than what is stated in this discussion.
If the efficacy of MB at this dosage were to hold true, a 1 fluid ounce bottle of MB at 1%(w/v) would provide sufficient MB at the concentration we are discussing for 3 doses taken daily for 5 years. The cost for that 1 fluid ounce (1 %) of MB sold as a Lab stain is $4. A medical prescription of MB, the same quantity costs $30. Either way MB would be an extremely cheap treatment for dementia, parkinsons or increasing one's longevity. Justifying MB to acquire a prescription would involve jumping through some hoops with one's doctor (a touchy matter). There will be those that will buy MB lab stain to avoid the trouble.
Frankly, it is totally astounding to imagine that such a relatively simple and inexpensive chemical could have such a profound effect on human physiology.
> Every drop size is different depending on the viscosity of the fluid > and size of the orifice. I measured 26 drops per ml from my dropper > with this solution. Your mileage may vary.
> 40000 mcg divided by 30 ml divided by 26 drops per ml equals 51 mcg/ > drop. > JLC
I guess everyone knows that a varient of Methylene blue is what makes up the apparently promising new UK Alzheimer’s drug, "Rember."
"...the aggregation of tau protein is autocatalytic: once it gets going, it's a cascade. They believe that methylene blue disrupts the aggregation, and even helps to dissociate existing aggregates. Once they're out in their monomeric forms, the helical tau fragments are degraded normally again, and the whole tau backup starts to clear out"
> On Aug 24, 11:05 am, windwatcher <windwatc...@mac.com> wrote:
> > methylene blue eye whites - sounds like something from Dune. The spice > > extended life and gave those who used it blued overall eyes. Sci fi > > predicts the future? If it extends life, everyone would use it.
> I wonder if the extremely diluted MB they describe is really what they > used in their experiments. This could have been stated in order to > prevent ordinary citizens from going out and buying MB and trying it > out. At such diluted levels as described in their paper, no one is > going to hurt themselves. And certainly, no one is going to end up > with blue eyed eyeballs at such an extremely low concentration.
> The concentrations as described in the Science Daily article - a few > rain drops diluted in two olympic sized pools, are what apparently was > applied to the "petri dish" samples of living cells. In order to gain > such a concentration in one's blood stream, one would obviously need a > higher ingested concentration. How much so I do not know. Anyone have > an idea? if one weighs 70kg and a human is 60% water by weight, then > there is 42 liters of water in one's body. This could be used to reach > a rough estimate of what one would need to ingest to reach the > concentrations described in the paper, however, the MG would not > necessarily be distributed evenly throughout the 42 liters of water > within one's body.
I've used this for quite a while. Dosage : Mix 0.1cc scoop (~40 mg) methylene blue in 30 ml (1 oz) dropper bottle of water. Delivers ~50 mcg/drop. I mix one drop in each of my three tea drinks, and one in the morning early - so 200 mcg/day total. Patent app info and excerpts follows. JLC
Diaminophenothiazine compositions and uses thereof
In one embodiment, the dosage is between 0.1-10.0 mcg/kg body weight/ day. In a preferred embodiment, the dosage achieves a blood concentration of about 1.0 to 100 nM of the diaminophenothiazine. In another embodiment, the dosage achieves a brain concentration of about 1.0 to 100 nM of the diaminophenothiazine. For a human weighing about 160 lbs and having about 5 liters of blood, the daily dose of methylene blue (mol. wt.=319) needed to achieve this concentration is about 1.6 to 160 mcg. The oral dosage is typically given one to three times daily for prolonged periods, usually for at least 30 days. In some cases, the oral dosage is administered chronically, i.e. regularly over a period of at least 3, preferably at least 6, more preferably at least 12 months, wherein the regularity is at least once, preferably at least 2-3 times, more preferably at least 7 times (daily) per week.
Long-Term Administration of diaminophenothiazines Improve Skin Elasticity and Activity Levels in Patients over 50.
A double blind, randomized, vehicle controlled study is conducted in 160 subjects ranging in ages from 50 to 65. Treatment groups are prescribed 25 mcg diaminophenothiazine (azure A, azure B, azure C, thionine, toluidine blue, methylene blue, new methylene blue, or 1-9-dimethyl methylene blue) tablets taken orally with meals 3 times daily. Clinical monitoring, subjective self assessment, objective measurement methods of skin elasticity, epidermal hydration and skin surface lipids are used to determine effects of each treatment at four visits during 24 weeks. Clinical monitoring includes wrinkle counts, measurement of wrinkle depth around the right eye, and nasolabial fold depth. Results demonstrate consistent efficacy of diaminophenothiazine treatment over placebo in counteracting different signs of aging in the skin and improving overall energy levels.
I suppose it could be purchased at a pet store since it is used as an antifungal tx for tropical fish. This would, of course, necessitate careful attention to concentration, etc. And who's to say the stuff sold in pet stores isn't simple the industrial dye version...
> Well that is a pretty small drop in comparison to what the wiki page > for a drop states. The wiki page lists several defined drop sizes > ranging from 50 to 80-odd micro-liters. I'll have to measure my > droppers drop size to determine the concentration to match the dosage > you state.
> Presently MB is very inexpensive (that could change but apparently its > production is not expensive and suppliers could adapt to demand). I do > not know if there is a difference in purity between MB sold by lab > material supplier intended as a dye, and MB meant for human > consumption such as for treatment of methemoglobinemia. Do you have > any idea if there is a difference and what impurities would exist in a > "lower grade" MB? And would you recommend not using MB sold as a lab > stain?
> A 1 fluid oz bottle at 1% (w/v) would need to be diluted by a factor > of about 7. The MB is at a low concentration. Any impurities would be > at a lower level. The toxicity of MB alone, using the Wikipedia > reference, appears to be on the order of 5 mg/kg of body weight. Such > a dosage is 5,000 times greater than what is stated in this > discussion.
> If the efficacy of MB at this dosage were to hold true, a 1 fluid > ounce bottle of MB at 1%(w/v) would provide sufficient MB at the > concentration we are discussing for 3 doses taken daily for 5 years. > The cost for that 1 fluid ounce (1 %) of MB sold as a Lab stain is $4. > A medical prescription of MB, the same quantity costs $30. Either way > MB would be an extremely cheap treatment for dementia, parkinsons or > increasing one's longevity. Justifying MB to acquire a prescription > would involve jumping through some hoops with one's doctor (a touchy > matter). There will be those that will buy MB lab stain to avoid the > trouble.
> Frankly, it is totally astounding to imagine that such a relatively > simple and inexpensive chemical could have such a profound effect on > human physiology.
>> Every drop size is different depending on the viscosity of the fluid >> and size of the orifice. I measured 26 drops per ml from my dropper >> with this solution. Your mileage may vary.
>> 40000 mcg divided by 30 ml divided by 26 drops per ml equals 51 mcg/ >> drop. >> JLC
Hmmm, not too enthusiastic about anything "homeopathic" but checked out the Homeopathic Laboratories website and am confused by what is meant in homeopathic terms when concentrations are expressed in units of 200c or 200x. How do these compare for instance to the dose that JC101 describes in his (her?) regimen of ~200 mcg/day? Loki
There is a concern I have regarding the studies in the news reports and the dosing you describe. The Oakland Hospital research on cultured cells translates in micro-grams of MB per day, however, the University of Aberdeen research with Alzheimer's patients involved doses of milli- grams per day. 60 milli-grams was the apparent optimal dosing revealed from the phase II study. My concern is that these news stories are using the letter "m" as in "mg" to represent micro-grams whereas if taken literally, one would interpret the doses as in milligrams, i.e. 1000 times greater dose. Does anyone know what the doses in the University of Aberdeen and TauRx really are? This is critical for those amongst us that would take a risk and test MB on their own. Unless MB is made a controlled substance, there is likely to be widespread experimenting with the chemical.
On Sat, 30 Aug 2008 10:17:12 -0700 (PDT), tellurian.l...@gmail.com wrote:
>There is a concern I have regarding the studies in the news reports >and the dosing you describe. The Oakland Hospital research on cultured >cells translates in micro-grams of MB per day, however, the University >of Aberdeen research with Alzheimer's patients involved doses of milli- >grams per day. 60 milli-grams was the apparent optimal dosing revealed >from the phase II study. My concern is that these news stories are >using the letter "m" as in "mg" to represent micro-grams whereas if >taken literally, one would interpret the doses as in milligrams, i.e. >1000 times greater dose. Does anyone know what the doses in the >University of Aberdeen and TauRx really are? This is critical for >those amongst us that would take a risk and test MB on their own. >Unless MB is made a controlled substance, there is likely to be >widespread experimenting with the chemical.
---cut and pasted---- Nausea, stomach upset, diarrhea, vomiting, or bladder irritation may occur. If any of these effects continue or become bothersome, tell your doctor.
This medication may cause your urine or stool to turn green-blue. This effect is harmless and will disappear when the medication is stopped.
Remember that your doctor has prescribed this medication because he or she has judged that the benefit to you is greater than the risk of side effects. Many people using this medication do not have serious side effects.
Tell your doctor immediately if any of these rare but very serious side effects occur: dizziness, fainting, high fever, fast/irregular/ pounding heartbeat, pale/blue skin color, unusual tiredness.
Seek immediate medical attention if this rare but very serious side effect occurs: chest pain.
A very serious allergic reaction to this drug is rare. However, seek immediate medical attention if you notice any symptoms of a serious allergic reaction, including: rash, itching, swelling, severe dizziness, trouble breathing.
This is not a complete list of possible side effects. If you notice other effects not listed above, contact your doctor or pharmacist. ---- end of cut and pasted ----
The information at the following link seems possibly suggestive that methylene blue occurs naturally with belladonna alkaloids??
On Aug 30, 12:55 am, "Les Shelley" <ksc...@mchsi.com> wrote:
> Hmmm, not too enthusiastic about anything "homeopathic" but checked out the > Homeopathic Laboratories website and am confused by what is meant in > homeopathic terms when concentrations are expressed in units of 200c or > 200x. How do these compare for instance to the dose that JC101 describes in > his (her?) regimen of ~200 mcg/day?
---------------------------------
I got my little glass vial of blue-tinted Homeopathic Methylene Blue pellets from Hyland / Homeopathic Labs.
They are 3x potency - (1x=1:10, 2x=1:100, 3x=1:1000)
As you can see, the "x" potencies are in the "real" macro amount world (and these pills are pale blue! Forget any "c" potencies). Indeed, there are many simple herbal and mineral OTC "1X" remedies that use the "homeopathic" designation - as opposed to a "supplement" one - so they could make specific medical claims. The traditional homeopathic indication of MB is for "rheumatism and spasms" and they recommend 3-6 pills under the tongue 3-4 times /day.
I don't know how the 1:1000 dilution in the small volume of the pellets translate into the dosages discussed here - the sublingual route may overcome some of the assimilation and brain barrier questions. I'm going the conservative route for now with 3/ once a day.
I also got a box of Cognisure (Colostrinin) - which are also relatively small glazed tablets for chewing and holding in he mouth. They give a peculiar, staggered dose schedule on the box that I read they arrived at thru clinic testing. I also got some quality, water- soluble cinnamon extract too.
I've just got these because I've recently became aware that I could be be a candidate for Lewy Body Dementia (perhaps w/ relatively young onset). While these items are promising for Alzheimer's Amyloids and Tau, rather than Lewy bodies, they also may help with Lewy's accompanying Parkinson's symptoms (Lewy bodies are also found in straight PD).
I've recently read that either Cognisure (or MB/Rember?) is now being tested on Lewy bodies. Regardless, the MB and the Cognisure seem to have many other anti-aging benefits and are not very expensive considering the small amounts for chronic usage.
> On Aug 30, 12:55 am, "Les Shelley" <ksc...@mchsi.com> wrote:
> > Hmmm, not too enthusiastic about anything "homeopathic" but checked out the > > Homeopathic Laboratories website and am confused by what is meant in > > homeopathic terms when concentrations are expressed in units of 200c or > > 200x. How do these compare for instance to the dose that JC101 describes in > > his (her?) regimen of ~200 mcg/day?
> ---------------------------------
> I got my little glass vial of blue-tinted Homeopathic Methylene Blue > pellets from Hyland / Homeopathic Labs.
> They are 3x potency - (1x=1:10, 2x=1:100, 3x=1:1000)
> As you can see, the "x" potencies are in the "real" macro amount world > (and these pills are pale blue! Forget any "c" potencies). Indeed, > there are many simple herbal and mineral OTC "1X" remedies that use > the "homeopathic" designation - as opposed to a "supplement" one - so > they could make specific medical claims. The traditional homeopathic > indication of MB is for "rheumatism and spasms" and they recommend 3-6 > pills under the tongue 3-4 times /day.
> I don't know how the 1:1000 dilution in the small volume of the > pellets translate into the dosages discussed here - the sublingual > route may overcome some of the assimilation and brain barrier > questions. I'm going the conservative route for now with 3/ once a > day.
> I also got a box of Cognisure (Colostrinin) - which are also > relatively small glazed tablets for chewing and holding in he mouth. > They give a peculiar, staggered dose schedule on the box that I read > they arrived at thru clinic testing. I also got some quality, water- > soluble cinnamon extract too.
> I've just got these because I've recently became aware that I could be > be a candidate for Lewy Body Dementia (perhaps w/ relatively young > onset). While these items are promising for Alzheimer's Amyloids and > Tau, rather than Lewy bodies, they also may help with Lewy's > accompanying Parkinson's symptoms (Lewy bodies are also found in > straight PD).
> I've recently read that either Cognisure (or MB/Rember?) is now being > tested on Lewy bodies. Regardless, the MB and the Cognisure seem to > have many other anti-aging benefits and are not very expensive > considering the small amounts for chronic usage.
> "3x potency" = ? mg or ug (micrograms) ??- Hide quoted text -
Well, unfortunantely it's apples and oranges :-)
The round, 1/8" lactose pellets are treated with MB/H2O solution of 1:1000. So there's no reliable way of extracting actual volume amount of the MB in each.
Pellets were more convenient, but I guess I'll look next into an actual water/grain alcohol solution bottle and assume each drop would be approx 1mg of fluid containing the 1:1000 (3x) solution.
It's convenient, presumably sterile, and definately bluish!
BTW, I took one additional pellet in the evening and couldn't sleep. There could have been a few others reasons for this, but it was definately unusual.
> On Sat, 30 Aug 2008 10:17:12 -0700 (PDT), tellurian.l...@gmail.com > wrote:
> >There is a concern I have regarding the studies in the news reports > >and the dosing you describe. The Oakland Hospital research on cultured > >cells translates in micro-grams of MB per day, however, the University > >of Aberdeen research with Alzheimer's patients involved doses of milli- > >grams per day. 60 milli-grams was the apparent optimal dosing revealed > >from the phase II study. My concern is that these news stories are > >using the letter "m" as in "mg" to represent micro-grams whereas if > >taken literally, one would interpret the doses as in milligrams, i.e. > >1000 times greater dose. Does anyone know what the doses in the > >University of Aberdeen and TauRx really are? This is critical for > >those amongst us that would take a risk and test MB on their own. > >Unless MB is made a controlled substance, there is likely to be > >widespread experimenting with the chemical.
Your concern is a great concern.. most of the articles are stating 60mg (60/ thousanths of a gram) three times daily and the 100 mg was ineffective.. Correctly it should be micrograms or 60/millionth of a gram! So anyone who is ingesting 60mg is getting an ineffective 1000 time overdose. from what I gather, a popular "ich" fish methylene blue is 2.303% w/v so that is 2.303 grams in 100cc of distilled purified water. Take a ml or 1/100th = 23 mg / ml (1ml = 1 dropperful) .. Take 2 ml or 2 dropperfuls = 46mg , place it in 30ml water and you get a dilution of 1.53 mg /ml. There 20 drops to a ml in a medicine dropper, so 1.53 mg divided by 20 = .075 mg which is 75 microgram- we only want 60mcg, so let's go back to the 2 dropperfuls X 60mcg/75mcg = 1.6 dropperful in 30 ml medicinal dropper bottle and qs to 30ml which now gives the 60mcg per DROP! three times daily. So if no one reads this blog there are going to be a lot of people taking and ineffective overdose. I guess that is why they make pharmacists. I have tried to correct this on a few articles but you have to register and that is blocked. so what do they say caveat emptor .. beware the buyer.
CLAYTON TOM, Reg. Pharmacist, UCSF School of Pharmacy '60. THIS IS FREE. I WILL BE CHARGING $10 FOR THIS ON MY WEBSITE www.4mRx where I will be giving pharmaceutical advise on off label uses. still under construction. make a note and check back later. in the meantime... 4meRxi...@gmail.com / cancunch...@gmail.com
|
